Targeted deletion of cellular DNA sequences
US8409861B2 · kind B2 · utility
89Cited by
26References
12Claims
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Key dates
| Filing date | Dec 15, 2005 |
| Grant date | Apr 2, 2013 |
| Priority date | — |
| Expiry date | Jan 5, 2028 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/81
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed herein are methods and compositions for targeted deletion of double-stranded DNA. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain, and polynucleotides encoding same. Methods for targeted deletion include introduction of such fusion proteins, or polynucleotides encoding same, into a cell such that two targeted cleavage events occur. Subsequent cellular repair mechanisms result in deletion of sequences between the two cleavage sites.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.