Use of triplex structure DNA in transferring nucleotide sequences

US8460678B2 · kind B2 · utility

Assignees

• INSTITUT PASTEUR · FR
• Institut National de la Sante et de la Recherche Medicale (INSERM) · FR
• CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE · FR

Inventors

• Pierre Charneau · Paris, FR
• Veronique Zennou · Paris, FR
• Hüseyin Firat · Paris, FR

Key dates

• Filing date: Sep 21, 2011
• Grant date: Jun 11, 2013
• Expiry date: Sep 21, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2840/203
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

The invention concerns a recombinant vector characterized in that it comprises a polynucleotide comprising a central initiation cis-active region (cPPT) and a termination cis-active region (CTS), the regions being of retroviral or retroviral-like origin, and the vector further comprising a predetermined nucleotide sequence (transgene or nucleotide sequence of interest) and retrotranscription regulating, expressing, and packaging signals of retroviral or retroviral-like origin.