Patent · US Active

Method for producing recombinant proteins with a constant content of pCO2 in the medium

US8460895B2 · kind B2 · utility

2Cited by

1References

13Claims

0Family size

Assignee

Hoffmann-La Roche Inc. · US

Inventors

Detlef Eisenkraetzer · Iffeldorf, DE
Jochen Gaetgens · Emmelshausen, DE
Alexander Jockwer · Eurasburg, DE
Christian Klinger · Penzberg, DE
Thomas Noll · Kipfenberg, DE
Barbara Bezdek-Sueess · Aesch, CH

Key dates

Filing date	Mar 11, 2009
Grant date	Jun 11, 2013
Priority date	—
Expiry date	Mar 11, 2029

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2500/02
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A method for the recombinant production of a polypeptide in a eukaryotic host cell modified in the citrate cycle is described, wherein the method comprises cultivating the eukaryotic host cell in a suitable medium under conditions which allow the expression of the polypeptide, wherein the content of dissolved CO2 (pCO2) in the medium is maintained at a constant value in the range of 10% to 20%.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.