Methods for preparing immunogenic conjugates

US8481048B2 · kind B2 · utility

Assignee

• The United States of America as Represented by The Secretary of the Department of Health and Human Services National Institutes of Health Office of Technology Transfer · US

Inventors

• Rachel Schneerson · Bethesda, US
• Joanna Kubler-Kielb · Rockville, US
• Fathy Majadly · Frederick, US
• Stephen H. Leppla · Bethesda, US
• John B. Robbins · New York, US
• Darrell T. Liu · Bethesda, US
• Joseph Shiloach · Rockville, US

Key dates

• Filing date: Oct 20, 2009
• Grant date: Jul 9, 2013
• Expiry date: Nov 23, 2030

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K2039/627
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods for making an immunogenic conjugate that includes a hapten or an antigen covalently linked to a carrier. The methods include reacting a first agent with a dihydrazide resulting in a hydrazine-modified first agent, wherein the first agent is a hapten, an antigen or a carrier; reacting a second agent with a benzaldehyde compound resulting in a benzaldehyde-modified second agent, wherein the second agent is a hapten, an antigen or a carrier, provided that the first agent or the second agent is a carrier; and reacting the hydrazine-modified first agent with the benzaldehyde-modified second agent resulting in an immunogenic conjugate comprising a hapten or an antigen covalently linked to a carrier via a hydrazone linkage.