Electrochemical polynucleotide detection comprising ligation
US8551697B1 · kind B1 · utility
7Cited by
2References
3Claims
0Family size
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Key dates
| Filing date | Dec 11, 2006 |
| Grant date | Oct 8, 2013 |
| Priority date | — |
| Expiry date | Jun 18, 2027 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6825
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed, for example, are methods comprising cleaving an uncleaved probe to form a cleaved oligonucleotide flap, forming a hybridization complex between the cleaved oligonucleotide flap, a bridging oligonucleotide, and a capture oligonucleotide that is immobilized on a surface, such that the oligonucleotide flap and the capture oligonucleotide are hybridized to immediately adjacent, complementary regions of the bridging oligonucleotide, ligating the oligonucleotide flap to the capture oligonucleotide to form an immobilized ligation product, and detecting the ligation product.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.