Method of loading sample into a microfluidic device
US8551698B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Aug 13, 2007 |
| Grant date | Oct 8, 2013 |
| Priority date | — |
| Expiry date | Aug 18, 2030 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/2575
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
A method comprising loading a sample into a microfluidic device which comprises plural sample chambers, subdividing the sample into a plurality of sample portions, such that respective sample portions are positioned in each of a plurality of the sample chambers, and subjecting the sample portions loaded into the respective sample chambers to at least a first amplification step. Each of the sample chambers has a respective volume such that if a sample portion positioned in the sample chamber comprises at least one molecule of a target nucleic acid, the target nucleic acid would attain a detectable concentration in the sample chamber after a single round of amplification.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.