Method for the production of insulin-like growth factor-1

US8552158B2 · kind B2 · utility

Assignee

• Hoffmann-La Roche Inc. · US

Inventors

• Stephan Fischer · Weilheim, DE
• Friederike Hesse · Munich, DE
• Hendrik Knoetgen · Penzberg, DE
• Kurt Lang · Penzberg, DE
• Joerg Thomas Regula · Munich, DE
• Christian Schantz · Penzberg, DE
• Andreas Schaubmar · Penzberg, DE

Key dates

• Filing date: Aug 29, 2007
• Grant date: Oct 8, 2013
• Expiry date: Jun 3, 2028

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/50
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Method for the production of IGF-I, characterized by cultivating a prokaryotic host cell comprising an expression vector containing a nucleic acid encoding a fusion protein comprising said IGF-I N-terminally linked to the C-terminus of a propeptide, whereby said propeptide ends C-terminally with amino acids -Y-Pro, wherein Y is selected from the group consisting of Pro, Pro-Ala, Pro-Gly, Pro-Thr, Ala-Pro, Gly-Pro, Thr-Pro, Arg-Pro, or Pro-Arg-Pro, recovering and cleaving said fusion protein with IgA protease, and recovering said IGF-I. IGF-I is useful for the treatment of neurodegenerative disorders like Alzheimer's Disease.