Infectious clone of human parvovirus B19 and methods

US8603784B2 · kind B2 · utility

Assignees

• The United States of America, as represented by the Secretary, Department of Health and Human Services · US
• INSTITUT NATIONAL DE LA RECHERCHE SCIENTIFIQUE · CA

Inventors

• Kevin E. Brown · London, US
• Ning Zhi · Rockville, US
• Peter Tijssen · Rue du Bois des Loges, CA
• Zoltan Zadori · Montréal, CA

Key dates

• Filing date: Sep 29, 2009
• Grant date: Dec 10, 2013
• Expiry date: Feb 27, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2750/14251
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to infectious clones of parvovirus B19, methods of cloning infectious B19 clones, and methods of cloning viral genomes that have secondary DNA structures that are unstable in bacterial cells. A B19 infectious clone and methods of producing B19 infectious clones are useful for producing infectious virus. Infectious virus is useful for identifying and developing therapeutically effective compositions for treatment and/or prevention of human parvovirus B19 infections.