Patent · US Active

Increased resolution microscopy

US8610086B2 · kind B2 · utility

6Cited by

6References

14Claims

0Family size

Assignee

Carl Zeiss Microscopy GmbH · DE

Inventors

Ralf Wolleschensky · Jena, DE
Ingo Kleppe · Jena, DE
Gerhard Krampert · Jena, DE
Michael Kempe · Jena, DE

Key dates

Filing date	Nov 14, 2009
Grant date	Dec 17, 2013
Priority date	—
Expiry date	Feb 22, 2030

Classification

Technology area (CPC G)Physics
CPC primaryG02B27/58
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

Method for spatially high-resolution luminescence microscopy in which label molecules in a sample are activated to emit luminescence radiation comprising activating only a subset of the label molecules in the sample, wherein activated label molecules have a distance to the closest activated molecules that is greater or equal to a length which results from a predetermined optical resolution, detecting the luminescence radiation, generating a frame from the luminescence radiation, identifying the geometric locations of the label molecules with a spatial resolution increased above the predetermined optical resolution, repeating the steps and forming a combined image, and controlling the acquisition of the several frames by evaluating at least one of the frames or a group of the frames and modifying at least one variable for subsequent repetitions of the steps of generating frames for combining into an image.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.