Preferential amplification of mRNA over DNA using chemically modified primers
US8614071B2 · kind B2 · utility
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7Claims
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Key dates
| Filing date | Dec 8, 2010 |
| Grant date | Dec 24, 2013 |
| Priority date | — |
| Expiry date | Sep 30, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a method, oligonucleotides, reaction mixtures and kits for the selective amplification of a messenger RNA target comprising an exon-exon junction, using an oligonucleotide that comprises at least one nucleotide modified at the exocyclic amino group.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.