Linear amplification of short nucleic acids
US8628925B2 · kind B2 · utility
1Cited by
3References
20Claims
0Family size
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Inventor
Key dates
| Filing date | Jun 6, 2011 |
| Grant date | Jan 14, 2014 |
| Priority date | — |
| Expiry date | Dec 22, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6853
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present teachings provide novel methods for amplifying short nucleic acids. In some embodiments, the present teachings provide novel methods for linearly amplifying a collection of micro RNAs by using temperature cycling during a reverse transcription reaction. The cycling can comprise at least 20 cycles of an annealing temperature segment of 10° C.-30° C., and a denaturation temperature segment of 35° C.-60° C. In some embodiments, the temperature cycled reaction can comprise an osmolyte.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.