Method for generating images with an expanded dynamic range and optical device for carrying out such a method, in particular a laser scanner microscope

US8633430B2 · kind B2 · utility

Assignee

• Carl Zeiss Microscopy GmbH · DE

Inventors

• Gunter Möhler · Jena, DE
• Dietmar Schmidt · Kahla, DE
• Oliver Holub · Jena, DE

Key dates

• Filing date: Mar 30, 2012
• Grant date: Jan 21, 2014
• Expiry date: Jul 19, 2032

Classification

• Technology area (CPC G): Physics
• CPC primary: G01J1/42
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Method and apparatus for generating an at least two-dimensional image of at least part of a sample. The method involves scanning the sample. Acquiring at least one light signal by an optoelectronic detector for different areas of the sample. Converting the light signal into an electrical signal. Distributing the electrical signal onto several parallel evaluation channels whose signal evaluations differ from each other so that their dynamic ranges are different. Generating a result signal in each evaluation channel. Selecting at least one of the result signals as a function of one of the result signals in order to generate the image for the sample range concerned. It is also possible to generate one intermediate result signal for each channel, typically from the respective actual result signal and one or more other sources. Thus the signal selection depending on both the result signals and the intermediate result signals are possible.