Molecular scaffolds for HIV-1 epitopes

US8637234B2 · kind B2 · utility

Assignees

• THE UAB RESEARCH FOUNDATION · US
• The Administrators of the Tulane Educational Fund · US
• The United States of America, as represented by the Secretary, Department of Health and Human Services · US

Inventors

• George M. Shaw · Birmingham, US
• James E. Robinson · Genval, BE
• Frederic Bibollet-Ruche · Birmingham, US
• Julie Decker · Birmingham, US
• Beatrice H. Hahn · Birmingham, US
• Peter Kwong · Washington, US

Key dates

• Filing date: Apr 8, 2005
• Grant date: Jan 28, 2014
• Expiry date: Aug 26, 2027

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2469/20
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Methods and compositions are provided for the use of an envelope polypeptide or a functional variant thereof from a lentivirus that is not HIV-1 as a molecular scaffold for HIV-1 epitopes. The HIV-1 epitopes can be recognized by HIV-1 binding antibodies, HIV-1 neutralizing antibodies and/or CD4-induced antibodies. Thus, methods are provided for detecting HIV-1 binding antibodies in a subject infected with HTV-1. Further provided are methods to determine an epitope for an HIV-1 binding antibody; methods to assay for an HIV-1 binding antibody; methods to identify a soluble CD4 mimic; methods to neutralize an non-HIV-1 virus; diagnostic assays to monitor HIV disease in a subject or to monitor the subject's response to immunization by a HIV vaccine; and methods to alter the neutralization potential of an HIV-1 derived CD4-induced antibody. Chimeric polypeptides, chimeric polynucleotides, kits, cells and viruses are also provided.