Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
US8637306B2 · kind B2 · utility
2Cited by
10References
23Claims
0Family size
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Key dates
| Filing date | Dec 9, 2009 |
| Grant date | Jan 28, 2014 |
| Priority date | — |
| Expiry date | Jul 23, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided are methods of making carrier polypeptide that include incorporating a first unnatural amino acid into a carrier polypeptide variant, incorporating a second unnatural amino acid into a target polypeptide variant, and reacting the first and second unnatural amino acids to produce the conjugate. Conjugates produced using the provided methods are also provided. In addition, orthogonal translation systems in methylotrophic yeast and methods of using these systems to produce carrier and target polypeptide variants comprising unnatural amino acids are provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.