Disposable device for analyzing a liquid sample containing a nucleic acid with a nucleic acid amplification apparatus
US8642293B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 5, 2007 |
| Grant date | Feb 4, 2014 |
| Priority date | — |
| Expiry date | Jan 14, 2029 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2300/0809
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The invention is directed to a disposable sample holding and processing device (1) dimensioned for being operated in a nucleic acid amplification apparatus for analyzing a liquid sample containing a nucleic acid by a nucleic acid amplification technique. The device (1) comprises chambers (15, 16) and channels (44) designed to perform the steps of capturing, amplification and detection of the nucleic acid within the device (1), and optionally also a lysis chamber (3). The binding chamber (15) contains a solid phase (14) for immobilization of a component of the sample to be analyzed, and the amplification chamber (16) is connected to the binding chamber (15) by a fluid channel (44). The device (1) comprises a rigid body (42) and at least one channel (44), the binding chamber (15) and the amplification chamber (16) are situated on side-surfaces (50) of the body (42), each of those side-surfaces (50), on which a channel (44), the binding chamber (15) or the amplification chamber (16) is situated, is covered by at least one wall (41) and these side-surfaces (50) are substantially vertical planes when the device (1) is operated in the nucleic acid amplification apparatus.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.