Ammonia-specific 5′-XMP aminase mutant
US8669078B2 · kind B2 · utility
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Key dates
| Filing date | Dec 14, 2006 |
| Grant date | Mar 11, 2014 |
| Priority date | — |
| Expiry date | Aug 29, 2029 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P19/32
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed herein are ammonia-specific 5′-XMP aminase mutants and a method for preparing the same. A mutation is introduced into the active site of glutamine-dependent catalysis in 5′-XMP aminase. The resulting 5′-XMP aminase mutant is devoid of the glutamine-dependent activity and specifically reacts with external ammonia in converting 5′-XMP into 5′-GMP. Thus, the ammonia-specific 5′-XMP aminase mutant is stabler within cells compared to the wild type, and can be useful in the industrial conversion of 5′-XMP into 5′-GMP.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.