Catalytic domains of beta(1,4)-galactosyltransferase I having altered metal ion specificity

US8703459B2 · kind B2 · utility

Assignee

• The United States of America, as represented by the Secretary, Department of Health and Human Services · US

Inventors

• Pradman K. Qasba · Bethesda, US
• Elizabeth Boeggeman · Bethesda, US
• Boopathy Ramakrishnan · Braintree, US

Key dates

• Filing date: Dec 6, 2004
• Grant date: Apr 22, 2014
• Expiry date: Jun 14, 2029

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07H5/06
• WIPO field: Organic fine chemistry
• WIPO sector: Chemistry

Abstract

Disclosed are mutants of galactosyltransferases that can catalyze formation of oligosaccharides in the presence of magnesium; mutants of galactosyltransferases having altered donor and acceptor specificity which can catalyze formation of oligosaccharides in the presence of magnesium; methods and compositions that can be used to synthesize oligosaccharides; methods for increasing the immunogenicity of an antigen; and methods to stabilize platelets.