DNA polymerases with increased 3′-mismatch discrimination

US8735120B2 · kind B2 · utility

Assignee

• Roche Molecular Systems, Inc. · US

Inventors

• Fred Reichert · Oakland, US
• Keith Bauer · Los Altos, US
• Thomas W. Myers · Dublin, US
• Nancy Schoenbrunner · Pleasanton, US
• Joseph San Filippo · Dublin, US

Key dates

• Filing date: Jun 17, 2011
• Grant date: May 27, 2014
• Expiry date: Feb 10, 2032

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02P20/52
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases.