Oligonucleotide compound and method for treating nidovirus infections

US8759307B2 · kind B2 · utility

Assignees

• Sarepta Therapeutics, Inc. · US
• THE SCRIPPS RESEARCH INSTITUTE · US

Inventors

• David A. Stein · Corvallis, US
• Richard K. Bestwick · Corvallis, US
• Patrick L. Iversen · Corvallis, US
• Benjamin Neuman · Brightwell-cum-Sotwell, GB
• Michael Buchmeier · Encinitas, US
• Dwight D. Weller · Corvallis, US

Key dates

• Filing date: Apr 25, 2008
• Grant date: Jun 24, 2014
• Expiry date: May 1, 2031

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2770/20022
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method and oligonucleotide compound for inhibiting replication of a nidovirus in virus-infected animal cells are disclosed. The compound (i) has a nuclease-resistant backbone, (ii) is capable of uptake by the infected cells, (iii) contains between 8-25 nucleotide bases, and (iv) has a sequence capable of disrupting base pairing between the transcriptional regulatory sequences in the 5′ leader region of the positive-strand viral genome and negative-strand 3′ subgenomic region. In practicing the method, infected cells are exposed to the compound in an amount effective to inhibit viral replication.