Method and arrangement for controlled actuation of a microscope, in particular of a laser scanning microscope

US8780443B2 · kind B2 · utility

Assignee

• Carl Zeiss Microscopy GmbH · DE

Inventors

• Ralf Engelmann · Jena, DE
• Joerg-Michael Funk · Arth, CH
• Bernhard Zimmerman · Großenhain, DE
• Ralph Netz · Jena, DE
• Frank Hecht · Weimar, DE

Key dates

• Filing date: Oct 13, 2009
• Grant date: Jul 15, 2014
• Expiry date: Aug 5, 2030

Classification

• Technology area (CPC G): Physics
• CPC primary: G02B27/0972
• WIPO field: Optics
• WIPO sector: Instruments

Abstract

Method for actuation control of a microscope, in particular of a Laser Scanning Microscope, in which, at least one first illumination light, preferably moving at least in one direction, as well as at least one second illumination light moving at least in one direction, illuminate a sample through a beam combiner, a detection of the light coming from the sample takes place, whereby, at least one part of the illumination light is generated through the splitting of the light from a common illuminating unit, characterized in that, by means of a common control unit, a controlled splitting into the first and the second illumination light takes place, in which the intensity of the first illuminating light, specified by the user or specified automatically, is assigned a higher priority (is prioritized) compared to the specified value for the second illumination light, and an adjustment for the second illumination light takes place until a maximum value is obtained, which is determined by the value specified for the first illumination light.