Patent · US Active

Miniaturized, high-throughput nucleic acid analysis

US8822158B2 · kind B2 · utility

12Cited by

20References

20Claims

0Family size

Assignee

Roche Molecular Systems, Inc. · US

Inventors

Thomas Froehlich · Ottikon, CH
Dieter Heindl · Munich, DE
Angelika Roesler · Sindelsdorf, DE
Tobias Heckel · Munich, DE

Key dates

Filing date	Jan 22, 2013
Grant date	Sep 2, 2014
Priority date	—
Expiry date	Jan 22, 2033

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6846
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention is directed to method for analyzing multiple nucleic acid molecules of interest comprising in the steps of (i) providing a plurality of beads, characterized in that each bead comprises at least two sequence specific amplification primers, further characterized in that at least one of the primers is bound to the bead via a cleavable linker, (ii) capturing the nucleic acid molecules of interest from a sample, (iii) clonally isolating the plurality of beads, (iv) cleaving the at least one primer, (v) clonally amplifying the nucleic acid thereby creating multiple amplification products, and (vi) analyzing the amplification products.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.