Microscope with light sheet illumination

US8848268B2 · kind B2 · utility

Assignee

• Carl Zeiss Microscopy GmbH · DE

Inventors

• Helmut Lippert · Jena, DE
• Matthias Wald · Jena, DE
• Michael Goelles · Jena, DE
• Robert Hauschild · Jena, DE

Key dates

• Filing date: Sep 17, 2010
• Grant date: Sep 30, 2014
• Expiry date: Sep 17, 2030

Classification

• Technology area (CPC G): Physics
• CPC primary: G02B26/08
• WIPO field: Optics
• WIPO sector: Instruments

Abstract

A family of microscopes include an illumination device which produces a planar light sheet along an illumination axis of an illumination beam path and a transverse axis normal to the illumination axis. A detection device detects light emitted from the sample region along an axis of detection of a detection beam path. The illumination and detection axes as well as the transverse axis and the axis of detection being oriented relative each other at an angle unequal to zero. A light sheet generator also produces rotationally symmetrical light and includes structure and control for rapidly scanning the sample region along the transverse axis. The illumination device includes a second light sheet generator having a first astigmatically active optical element with at least one astigmatic lens for producing a static sheet of light. Selection elements used to select either the first or the second light sheet or both together to produce the sheet of light.