Reengineering mRNA primary structure for enhanced protein production
US8853179B2 · kind B2 · utility
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Key dates
| Filing date | Feb 24, 2010 |
| Grant date | Oct 7, 2014 |
| Priority date | — |
| Expiry date | May 28, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2320/53
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Described herein are rules to modify natural mRNAs or to engineer synthetic mRNAs to increase their translation efficiencies. These rules describe modifications to mRNA coding and 3′ UTR sequences intended to enhance protein synthesis by: 1) decreasing ribosomal diversion via AUG or non-canonical initiation codons in coding sequences, and/or 2) by evading miRNA-mediated down-regulation by eliminating one or more miRNA binding sites in coding sequences.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.