Patent · US Active

Prokaryotic expression construct

US8877895B2 · kind B2 · utility

0Cited by

1References

9Claims

0Family size

Assignee

Hoffmann-La Roche Inc. · US

Inventors

Adelbert Grossmann · Murnau am Staffelsee, DE
Friederike Hesse · Munich, DE
Erhard Kopetzki · Penzberg, DE
Wilma Lau · Munich, DE
Christian Schantz · Penzberg, DE

Key dates

Filing date	Aug 25, 2011
Grant date	Nov 4, 2014
Priority date	—
Expiry date	Aug 25, 2031

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2319/70
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A pro-polypeptide which is useful for the expression of a polypeptide of interest in a prokaryotic cell. Therefore the pro-polypeptide is fused to the N-terminus of the polypeptide of interest. The pro-polypeptide as reported herein provides for improved expression yields and improves the handling of the fusion polypeptide (downstream processing, purification). For example, efficient endotoxin removal is effected while the protein of interest comprising the pro-polypeptide is bound e.g. to an affinity chromatography material. Thereafter the pro-polypeptide can efficiently be cleaved from the polypeptide of interest by the incorporated protease cleavage site with the cognate protease.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.