CRISPR-Cas nickase systems, methods and compositions for sequence manipulation in eukaryotes
US8889356B2 · kind B2 · utility
373Cited by
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Key dates
| Filing date | Feb 18, 2014 |
| Grant date | Nov 18, 2014 |
| Priority date | — |
| Expiry date | Feb 18, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2310/531
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.