Method for evaluating fluorescence correlation spectroscopy measurement data

US8892400B2 · kind B2 · utility

Assignee

• Carl Zeiss Microscopy GmbH · DE

Inventors

• Stephan Wagner-Conrad · Jena, DE
• Yauheni Novikau · Jena, DE
• Klaus Weisshart · Jena, DE

Key dates

• Filing date: Mar 29, 2011
• Grant date: Nov 18, 2014
• Expiry date: May 24, 2033

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N21/6458
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

With the different methods of fluorescence correlation spectroscopy, physical and biological transport processes in or between cells in the microscopic range, for example diffusion processes, can be analyzed. For this purpose, correlations of the fluorescence measurement data are determined for different sample regions and mathematical transport models are adapted thereto. Erroneous fluorescence correlation analyses were previously identified on the basis of the properties of the adapted model function parameters and were discarded. The a-priori knowledge necessary for the identification had to be obtained in time-consuming series of tests. With the invention, sample properties can be determined in a simpler, quicker and more exact way from fluorescence correlations. A suitability degree for one or more regions of the sample is determined for a correlation evaluation, describing quantitatively the information content of the respective region, or the error to be expected from a correlation evaluation, and can thus already be used before a correlation evaluation as a criterion for filtering/selecting the respective region. In this way, elaborate correlation calculations can be dispen…