Patent · US Active

Apparatus, especially microscope, for the analysis of samples

US8908174B2 · kind B2 · utility

0Cited by

2References

3Claims

0Family size

Assignee

Carl Zeiss Microscopy GmbH · DE

Inventors

Michael Kempe · Jena, DE
Gerhard Krampert · Jena, DE
Ingo Kleppe · Jena, DE
Ralf Wolleschensky · Jena, DE

Key dates

Filing date	Sep 22, 2009
Grant date	Dec 9, 2014
Priority date	—
Expiry date	Mar 27, 2031

Classification

Technology area (CPC G)Physics
CPC primaryG01N2021/6417
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

A microscope device which has a diffraction-limited resolution volume, with multiple dye molecules that can be switched between different states, at least one of which is fluorescent. The fluorescence is focused using an objective lens and is imaged onto a spatially resolving detector. In at least one portion of the sample, the dye molecules have a distribution density that is greater than the inverse of the diffraction-limited resolution volume. One or more light sources are provided for emitting a switching radiation in order to switch a first subset of the dye molecules in the sample, and for emitting an excitation radiation in order to excite the first subset of dye molecules. A phase mask which generates a light distribution having an at least partially limited local minimum radiation on the detector plane is provided in the beam path, preferably in the detection beam path.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.