Robust expression of a bioactive mammalian protein in Chlamydomonas chloroplast
US8932576B2 · kind B2 · utility
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Key dates
| Filing date | Jan 27, 2010 |
| Grant date | Jan 13, 2015 |
| Priority date | — |
| Expiry date | Oct 25, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8257
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and compositions are disclosed to engineer chloroplast comprising heterologous mammalian genes via a direct replacement of chloroplast Photosystem II (PSII) reaction center protein coding regions to achieve expression of recombinant protein above 5% of total protein. When algae is used, algal expressed protein is produced predominantly as a soluble protein where the functional activity of the peptide is intact. As the host algae is edible, production of biologics in this organism for oral delivery of proteins/peptides, especially gut active proteins, without purification is disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.