Method of detecting botulinum neurotoxin and antibodies that neutralize botulinum neurotoxin action
US8940477B2 · kind B2 · utility
2Cited by
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16Claims
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Key dates
| Filing date | Nov 10, 2008 |
| Grant date | Jan 27, 2015 |
| Priority date | — |
| Expiry date | Aug 1, 2032 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/33
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A highly sensitive method of analyzing a sample for the presence or activity of botulinum neurotoxin (BoNT) or antibodies specific for botulinum neurotoxin is disclosed. In one embodiment, the method comprises the steps of preparing primary non-human mammalian or avian spinal cord cells, and exposing the cells to a test sample, in parallel with a control sample, and examining the extent of cleavage of the intracellular neuronal target protein in both the test and control sample.
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