Reverse genetics of negative-strand RNA viruses in yeast
US8980634B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jan 30, 2009 |
| Grant date | Mar 17, 2015 |
| Priority date | — |
| Expiry date | Oct 26, 2030 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2770/24134
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention relates to a methodology for the generation of infectious ribonucleoparticles (RNPs) of negative-strand RNA viruses, and in particular of non-segmented negative-strand RNA viruses in yeast, especially in budding yeast. Accordingly, the patent application relates to a recombinant yeast strain suitable for the rescue of infectious non-segmented negative-strand RNA virus particles or infectious virus-like particles. The invention also relates to the use of the recombinant yeast to prepare vaccine seed and to the use of the produced RNPs or RNPs-like to prepare vaccine formulations. It also concerns the use of the recombinant yeast for the screening of libraries of DNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.