Patent · US Active

Structurally designed shRNAs

US8993532B2 · kind B2 · utility

27Cited by

5References

19Claims

0Family size

Assignee

Cold Spring Harbor Laboratory · US

Inventors

Gregory J. Hannon · Huntington, US
Sihem Cheloufi · Boston, US

Key dates

Filing date	Apr 22, 2011
Grant date	Mar 31, 2015
Priority date	—
Expiry date	Apr 22, 2031

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2320/30
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Provided is an improved design of shRNA based on structural mimics of miR-451 precursors. These miR-451 shRNA mimics are channeled through a novel small RNA biogenesis pathway, require AGO2 catalysis and are processed by Drosha but are independent of DICER processing. This miRNA pathway feeds active elements only into Ago2 because of its unique catalytic activity. These data demonstrate that this newly identified small RNA biogenesis pathway can be exploited in vivo to produce active molecules.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.