DNA polymerases with improved activity

US9017979B2 · kind B2 · utility

Assignee

• Roche Molecular Systems, Inc. · US

Inventors

• Keith Bauer · Los Altos, US
• Thomas W. Myers · Dublin, US
• Fred Reichert · Oakland, US
• Joseph San Filippo · Dublin, US
• Rachel Shahinian · Alameda, US
• Shawn Suko · El Sobrante, US

Key dates

• Filing date: Apr 10, 2012
• Grant date: Apr 28, 2015
• Expiry date: Feb 25, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y207/07049
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed are DNA polymerases having increased reverse transcriptase efficiency, mismatch tolerance, extension rate and/or tolerance of RT and polymerase inhibitors relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases.