Heat-resistant newcastle disease virus live vaccine vector system and use thereof

US9051584B2 · kind B2 · utility

Assignee

• INSTITUTE OF ANIMAL HUSBANDRY AND VETERINARY SCIENCES, HUBEI ACADEMY OF AGRICULTURAL SCIENCES · CN

Inventors

• Guoyuan Wen · 红钢城街道, CN
• Huabin Shao · Shanghai, CN
• Jun Yang · Beijing, CN
• Honglin Wang · Hikone, JP
• Qingping Luo · Beijing, CN
• Rongrong Zhang · Zhejiang, CN
• Diyun Ai · 红钢城街道, CN
• Ling Luo · Hangzhou City, CN
• Yu-Ming Shang · Beijing, CN
• Jing Guo · Beijing, CN
• Chen Chen · 安丰镇, CN

Key dates

• Filing date: Jun 24, 2013
• Grant date: Jun 9, 2015
• Expiry date: Jun 24, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N2760/18151
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A heat-resistant NDV live vaccine vector system includes a transcription plasmid, three helper plasmids, and host cells. The transcription plasmid is constructed by through cloning complete genomic cDNA of a heat-resistant NDV vaccine strain to a pBR322 vector. The three helper plasmids are constructed by cloning sequences coding nucleoprotein (NP), phosphoprotein, large polymerase protein of a heat-resistant NDV vaccine strain respectively to pcDNA3.1 vectors. A recombinant NDV artificially obtained by cotransfacting host cells with the transcription plasmid and the three helper plasmids shows heat-resistance.