Compositions and methods using split polymerases
US9085762B2 · kind B2 · utility
11Cited by
1References
17Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 31, 2007 |
| Grant date | Jul 21, 2015 |
| Priority date | — |
| Expiry date | Apr 24, 2031 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/1252
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to compositions and methods utilizing split polymerase enzymes composed of at least two discrete polypeptides that stably associate to form a single polymerase. The invention further relates to nucleic acid constructs for expressing the split polymerases of the invention, and methods for using the split polymerases of the invention. The enzymes of the invention are useful in many applications calling for the detectable labeling of nucleic acids and are particularly useful in quantitative PCR (QPCR) and DNA sequencing applications.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.