Orthogonal translation components for the in vivo incorporation of unnatural amino acids

US9102932B2 · kind B2 · utility

Assignee

• THE SCRIPPS RESEARCH INSTITUTE · US

Inventors

• Peter G. Schultz · Village of La Jolla, US
• Lital Alfonta · San Diego, US
• Johnathan R. Chittuluru · San Diego, US
• Alexander Deiters · Raleigh, US
• Dan Groff · Oakland, US
• Daniel Summerer · Konstanz, DE
• Meng-Lin Tsao · Merced, US
• Jiangyun Wang · San Diego, US
• Ning Wu · Boston, US
• Jianming Xie · San Diego, US
• Huaqiang Zeng · San Diego, US
• Mohammad R. Seyedsayamdost · Princeton, US
• James Petrie Turner · San Diego, US

Key dates

• Filing date: Jun 8, 2012
• Grant date: Aug 11, 2015
• Expiry date: Sep 18, 2032

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/02
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetase that can incorporate unnatural amino acid into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.