Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
US9109022B2 · kind B2 · utility
1Cited by
10References
21Claims
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Key dates
| Filing date | Oct 28, 2013 |
| Grant date | Aug 18, 2015 |
| Priority date | — |
| Expiry date | Oct 28, 2033 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/02
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided are methods of making carrier polypeptide that include incorporating a first unnatural amino acid into a carrier polypeptide variant, incorporating a second unnatural amino acid into a target polypeptide variant, and reacting the first and second unnatural amino acids to produce the conjugate. Conjugates produced using the provided methods are also provided. In addition, orthogonal translation systems in methylotrophic yeast and methods of using these systems to produce carrier and target polypeptide variants comprising unnatural amino acids are provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.