MGMT-based method for obtaining high yields of recombinant protein expression
US9109219B2 · kind B2 · utility
7Cited by
2References
17Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 9, 2011 |
| Grant date | Aug 18, 2015 |
| Priority date | — |
| Expiry date | Dec 17, 2031 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to a novel enhancer of protein production in host cells. It discloses a vector for expressing recombinant proteins in these cells, comprising a nucleotide sequence encoding a) a secretion peptidic signal, b) a 6-methylguanine-DNA-methyltransferase enzyme (MGMT, EC 2.1.1.63), a mutant or a catalytic domain thereof, and c) a recombinant protein. Said MGMT enzyme is preferably the so-called SNAP protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.