Patent · US Active

Sequence dependent aggregation

US9150641B2 · kind B2 · utility

3Cited by

2References

6Claims

0Family size

Assignee

Hoffmann-La Roche Inc. · US

Inventors

Hubert Kettenberger · Munich, DE
Stefan Klostermann · Schwanau, DE
Ulrich Kohnert · Habach, DE
Sebastian Neumann · Weilheim, DE

Key dates

Filing date	Dec 17, 2010
Grant date	Oct 6, 2015
Priority date	—
Expiry date	May 30, 2031

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2317/567
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Herein is reported a method for reducing the aggregation of an immunoglobulin in solution comprising the steps of i) comparing the amino acid sequence of the fourth framework region of the heavy chain of an antibody with a reference or germline sequence and determining whether one or more threonine residues and/or serine residues have been replaced by a different amino acid residue, and ii) modifying the amino acid sequence of the immunoglobulin by reverting the exchanged threonine residues and/or serine residues back to threonine or serine of the reference or germline sequence and thereby reducing the aggregation of an immunoglobulin in solution.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.