Process for preparing polysaccharide-protein conjugate vaccines

US9173931B2 · kind B2 · utility

Assignees

• The United States of America, as represented by the Secretary, Department of Health and Human Services · US
• Fiocruz · BR

Inventors

• Ellen Jessouroun · Rio de Janeiro, BR
• Ivna Alana Freitas Brasileiro Da Silveira · Rio de Janeiro, BR
• Renata Chagas Bastos · Rio de Janeiro, BR
• Carl E. Frasch · Rockville, US
• Che-Hung Robert Lee · Silver Spring, US

Key dates

• Filing date: Aug 6, 2004
• Grant date: Nov 3, 2015
• Expiry date: Jul 30, 2026

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02A50/30
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Methods for the manufacture of polysaccharide-protein conjugate vaccines at high yield are provided. The methods involve reaction of a hydrazide group on one reactant with an aldehyde group on the other reactant. The reaction proceeds rapidly with a high conjugation efficiency. Simplified purification processes can be employed to separate the conjugate product from the unconjugated protein and polysaccharide and other small molecule by-products.