Optimized method for antibody capturing by mixed mode chromatography

US9206227B2 · kind B2 · utility

Assignee

• Hoffmann-La Roche Inc. · US

Inventors

• Roberto Falkenstein · Munich, DE
• Thorsten Lemm · Kochel am See, DE
• Markus Strasser · Penzberg, DE
• Hidenari Yamada · Tokyo, JP

Key dates

• Filing date: Nov 2, 2011
• Grant date: Dec 8, 2015
• Expiry date: Jul 6, 2032

Classification

• Technology area (CPC —): General

Abstract

Herein is reported a method for the purification of an antibody directly captured from clarified cell culture supernatants using Streamline CST and/or Capto MMC, wherein especially product related (aggregates and fragments) and process related impurities (host cell protein, media components) could efficiently be removed, resulting in a preparation with a purity comparable to classical protein A affinity chromatography.