Human monoclonal antibodies specific for glypican-3 and use thereof

US9206257B2 · kind B2 · utility

Assignee

• The United States of America, as represented by the Secretary, Department of Health and Human Services · US

Inventors

• Mitchell Ho · Urbana, US
• Mingqian Feng · Rockville, US
• Dimiter S. Dimitrov · Frederick, US
• Wei Gao · Rockville, US

Key dates

• Filing date: Apr 19, 2012
• Grant date: Dec 8, 2015
• Expiry date: Apr 19, 2032

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2400/40
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Described herein is the identification of human monoclonal antibodies that bind GPC3 or heparan sulfate (HS) chains on GPC3 with high affinity. The antibodies described herein are capable of inhibiting HCC cell growth and migration. Provided are human monoclonal antibodies specific for GPC3 or HS chains on GPC3, including immunoglobulin molecules, such as IgG antibodies, as well as antibody fragments, such as single-domain VH antibodies or single chain variable fragments (scFv). Further provided are compositions including the antibodies that bind GPC3 or HS chains on GPC3, nucleic acid molecules encoding these antibodies, expression vectors comprising the nucleic acids, and isolated host cells that express the nucleic acids. Methods of treating cancer and/or inhibiting tumor growth or metastasis are also provided. Further provided are methods of detecting cancer in a subject and confirming a diagnosis of cancer in a subject.