Alpha 1-3 N-galactosylstransferase with altered donor specificities, compositions and methods of use

US9222080B2 · kind B2 · utility

Assignee

• The United States of America, as represented by the Secretary, Department of Health and Human Services · US

Inventors

• Pradman K. Qasba · Bethesda, US
• Boopathy Ramakrishnan · Braintree, US
• Elizabeth Boeggeman · Bethesda, US
• Marta Pasek · Frederick, US

Key dates

• Filing date: Apr 11, 2013
• Grant date: Dec 29, 2015
• Expiry date: Apr 11, 2033

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12P21/005
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The invention generally features compositions and methods based on the structure-based design of alpha 1-3 N-Acetylgalactosaminyltransferase (alpha 3 GalNAc-T) enzymes from alpha 1-3galactosyltransferase (a3Gal-T) that can transfer 2′-modified galactose from the corresponding UDP-derivatives due to substitutions that broaden the alpha 3Gal-T donor specificity and make the enzyme a3 GalNAc-T.