Patent · US Active

Allele-specific amplification of nucleic acids

US9238832B2 · kind B2 · utility

11Cited by

9References

16Claims

0Family size

Assignee

Roche Molecular Systems, Inc. · US

Inventors

Stephen G. Will · Oakland, US
Alison Tsan · Castro Valley, US
Nicolas Newton · Oakland, US

Key dates

Filing date	Dec 8, 2010
Grant date	Jan 19, 2016
Priority date	—
Expiry date	Apr 2, 2032

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q2600/166
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, having an internally-placed selective nucleotide complementary to only one variant of the target sequence wherein the allele-specific oligonucleotide is extended by a nucleic acid polymerase predominantly or exclusively when hybridized to the variant of the target sequence for which it has said complementary selective nucleotide.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.