Microscope having light sheet illumination of a sample region

US9239454B2 · kind B2 · utility

Assignee

• Carl Zeiss Microscopy GmbH · DE

Inventors

• Helmut Lippert · Jena, DE
• Matthias Wald · Jena, DE
• Michael Goelles · Jena, DE
• Robert Hauschild · Jena, DE
• Wolfgang Bathe · Jena, DE

Key dates

• Filing date: Sep 17, 2010
• Grant date: Jan 19, 2016
• Expiry date: Nov 9, 2030

Classification

• Technology area (CPC G): Physics
• CPC primary: G02B26/0891
• WIPO field: Optics
• WIPO sector: Instruments

Abstract

A microscope including an illumination device providing a light sheet illuminating a sample region, said sheet having a planar extension along an illumination axis of an illumination beam path and a transverse axis lying normal to the illumination axis. A detection device detects light emitted from the sample region on a detection axis the illumination axis and detection axis as well as the transverse axis and the detection axis being oriented relative each other at an angle unequal zero. The detection device has a detection lens system arranged in the detection beam path and splitting means for splitting the detection beam path into two beam sub-paths. A dichroic beam splitter in the infinity region of the surface detectors is about 3 mm thick. Wobble plate(s) disposed orthogonal to each other relative to the detection axis arranged in one of the two beam sub-paths so measured values can be automatically superimposed.