Patent · US Active

Method and apparatus for investigating a sample with regard to the lifetime of an excited state

US9274057B2 · kind B2 · utility

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20Claims

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Assignee

LEICA MICROSYSTEMS CMS GMBH · DE

Inventors

Juergen Schneider · Freinsheim, DE
Bernd Widzgowski · Dossenheim, DE
Jochen Sieber · Mannheim, DE
Wernher Fouquet · Mannheim, DE
Lars Friedrich · Weinheim, DE
Arnold Giske · Sandhausen, DE
Lioba Kuschel · Mannheim, DE

Key dates

Filing date	Apr 19, 2013
Grant date	Mar 1, 2016
Priority date	—
Expiry date	Apr 19, 2033

Classification

Technology area (CPC G)Physics
CPC primaryG01N2201/06113
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

The invention relates to a method for investigating a sample with regard to the lifetime of an excited state, in particular a fluorescence lifetime, and/or with regard to a property of a sample which is correlated with a lifetime of an excited state, in particular with a fluorescence lifetime, a sample region being illuminated with a sequence of excitation light pulses. The method is characterized in that the light quantity and/or number of photons of the detected light, in particular fluorescent light, proceeding from the sample region is measured temporally between the excitation light pulses exclusively within a detection time window in each case, at least two detection time windows having different temporal lengths.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.