Patent · US Active

RNAi sequence-independent modification formats, and stabilized forms thereof

US9284551B2 · kind B2 · utility

27Cited by

38References

7Claims

0Family size

Assignee

Applied Biosystems, LLC · US

Inventors

Nitin Puri · Austin, US
Irudaya Charles · Austin, US
Susan Magdaleno · Austin, US
Alexander Vlassov · Austin, US
Chris Burnett · Austin, US

Key dates

Filing date	Mar 1, 2013
Grant date	Mar 15, 2016
Priority date	—
Expiry date	Mar 1, 2033

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2320/53
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Modification formats having modified nucleotides are provided for siRNA. Short interfering RNA having modification formats and modified nucleotides provided herein reduce off-target effects in RNA interference of endogenous genes. Further modification formatted siRNAs are demonstrated to be stabilized to nuclease-rich environments. Unexpectedly, increasing or maintaining strand bias, while necessary to maintain potency for endogenous RNA interference, is not sufficient for reducing off-target effects in cell biology assays.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.