Detecting single nucleotide polymorphism using hydrolysis probes with 3′ hairpin structure
US9297033B2 · kind B2 · utility
3Cited by
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6Claims
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Key dates
| Filing date | Dec 13, 2013 |
| Grant date | Mar 29, 2016 |
| Priority date | — |
| Expiry date | Feb 12, 2034 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/156
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for the rapid detection of the presence or absence of a SNP in a target nucleic acid in a sample are described. The methods can include performing an amplifying step, a hybridizing step utilizing a SNP specific hydrolysis probe including a hairpin structure toward the 3′ end, and a detecting step. Furthermore, SNP specific hydrolysis probe including a hairpin structure toward the 3′ end, along with kits are provided that are designed for the detection of a SNP in a target nucleic acid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.