Single cell analysis by polymerase cycling assembly
US9347099B2 · kind B2 · utility
1Cited by
146References
20Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jun 3, 2013 |
| Grant date | May 24, 2016 |
| Priority date | — |
| Expiry date | Oct 20, 2033 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A90/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention provides a method of making measurements on individual cells of a population, particularly cells that have identifying nucleic acid sequences, such as lymphoid cells. In one aspect, the invention provides a method of making multiparameter measurements on individual cells of such a population by carrying out a polymerase cycling assembly (PCA) reaction to link their identifying nucleic acid sequences to other cellular nucleic acids of interest. The fusion products of such PCA reaction are then sequenced and tabulated to generate multiparameter data for cells of the population.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.