Method for biotechnological production of dihydrochalcones

US9359622B2 · kind B2 · utility

Assignee

• SYMRISE AG · DE

Inventors

• Jens-Michael Hilmer · Ankum, DE
• Egon Gross · Holzminden, DE
• Gerhard Krammer · Holzminden, DE
• Jakob Ley · Holzminden, DE
• Mechthild Gall · Greifswald, DE
• Uwe Bornscheuer · Greifswald, DE
• Maren Thomsen · Greifswald, DE
• Christin Peters · Greifswald, DE
• Patrick Jonczyk · Hannover, DE
• Sascha Beutel · Hannover, DE
• Thomas Scheper · Hannover, DE

Key dates

• Filing date: Jul 30, 2013
• Grant date: Jun 7, 2016
• Expiry date: Sep 16, 2033

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y02P20/52
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method for production of a dihydrochalcone, especially of phloretin, using a transgenic microorganism, containing a nucleic acid section (a), comprising or consisting of a gene coding for a bacterial chalcone isomerase, and/or a nucleic acid section (a′), comprising or consisting of a gene coding for a plant chalcone isomerase, and a nucleic acid section (b), comprising or consisting of a gene coding for a bacterial enoate reductase, corresponding transgenic microorganisms, containing a nucleic acid section (a), comprising or consisting of a gene coding for a bacterial chalcone isomerase, and/or a nucleic acid section (a′), comprising or consisting of a gene coding for a plant chalcone isomerase, and/or a nucleic acid section (b), comprising or consisting of a gene coding for a bacterial enoate reductase, and host cells, containing one or more identical or different such vectors.