Patent · US Active

Primers with modified phosphate and base in allele-specific PCR

US9382581B2 · kind B2 · utility

10Cited by

5References

6Claims

0Family size

Assignee

Roche Molecular Systems, Inc. · US

Inventors

Stefanie Froehner · Penzberg, DE
Dieter Heindl · Munich, DE
Dirk Kessler · Peiting, DE
Nancy Schoenbrunner · Pleasanton, US
Alison Tsan · Castro Valley, US

Key dates

Filing date	Nov 27, 2013
Grant date	Jul 5, 2016
Priority date	—
Expiry date	Jul 11, 2034

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12Q1/6858
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The present invention includes a method of allele-specific amplification, utilizing an allele-specific oligonucleotide, at least partially complementary to more than one variant of the target sequence, but having at least one selective nucleotide complementary to only one variant of the target sequence and incorporating both a nucleotide with a base covalently modified at the exocyclic amino group and a modified phosphate.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.